Adipose mesenchymal stem cells combined with platelet-rich plasma accelerate diabetic wound healing by modulating the Notch pathway

Abstract Background Diabetic foot ulceration is a serious chronic complication of diabetes mellitus characterized by high disability, mortality, and morbidity. Platelet-rich plasma (PRP) has been widely used for diabetic wound healing due to its content growth factors. However, application limited the rapid degradation The present study aimed evaluate efficacy combined adipose-derived mesenchymal stem cells (ADSCs) PRP therapy in promoting relation Notch signaling pathway. Methods Albino rats were allocated into 6 groups [control (unwounded), sham (wounded but non-diabetic), diabetic, PRP-treated, ADSC-treated, PRP+ADSCs-treated groups]. effect individual was evaluated assessing closure rate, epidermal thickness, dermal collagen, angiogenesis. Moreover, gene protein expression key elements pathway (Notch1, Delta-like canonical ligand 4 (DLL4), Hairy Enhancer Split-1 (Hes1), Hey1, Jagged-1), angiogenic marker (vascular endothelial factor stromal cell-derived 1) (EPSCs) related (ß1 Integrin) assessed. Results Our data showed better PRP+ADSCs compared their use after 7 14 days as caused reepithelialization granulation tissue formation with marked increase area percentage significantly downregulated, EPSC proliferation recruitment enhanced other treated groups. Conclusions These demonstrated that ADSCs accelerated wounds induced experimentally via modulating pathway, angiogenesis proliferation.